mock-env-aligned

Oligotype GC in "mock-env-aligned"

Page that explains diversity within an oligotype.

Unique Sequences Curve

» Abundance curve of unique sequences within GC, along with entropy values associated with these new set of alignments.

Distribution Among Samples

» Graphs below aim to give an idea about the distribution of unique sequences within GC among all samples.

This graph is here to remind the total number of reads for each sample that underwent the oligotyping analysis:

This graph shows the actual counts and distribution of unique sequences concealed within GC. By hovering over dots on the graph you can see the distribution of the first 20 sequences. If there are cases where the number of unique read #1 is competing with the number of other unique reads, it would mean that there is too much diversity trapped in this oligotype and needs to be decomposed by increasing the number of components. Sequences section below might help you decide which components might be appropriate to include.

This graph is similar to the one above, but normalized per sample by percent. Again, this should give you an idea about the concealed diversity.

Sequences

» First 20 Unique sequences

b GC ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||

715 GC b GT-TG-AAAAA-GTT-GGTG-GTG-AAAT-CCCAGA-GCTTAA-CT-CTGGAAC-TG-CCATC-AAAAC-TTTTCAG-CTA-GAGTAT-G-ATAGAGG-AAAGCAGAA-TTTCTAGT-G-TAGA-G-GTG-AAAT-TCG-T-AG-A-TATTA-G-AAA-GAATACCA-ATTG-CGA-AGGCAGC-TTTCT-GGATCATTACTGACA-C-TGAGGA-ACG-AAAGC-AT-GGGTAGCG-AAG-AGGA

21 GC b GT-TG--AAAA-GTT-GGTG-GTG-AAAT-CCCAGA-GCTTAA-CT-CTGGAAC-TG-CCATC-AAAAC-TTTTCAG-CTA-GAGTAT-G-ATAGAGG-AAAGCAGAA-TTTCTAGT-G-TAGA-G-GTG-AAAT-TCG-T-AG-A-TATTA-G-AAA-GAATACCA-ATTG-CGA-AGGCAGC-TTTCT-GGATCATTACTGACA-C-TGAGGA-ACG-AAAGC-AT-GGGTAGCG-AAG-AGGA

14 GC b GT-TG-AAAAA-GTT-GGTG-GTG-AAAT-CCCAGA-GCTTAA-CT-CTGGAAC-TG-CCATC-AAAAC--TTTCAG-CTA-GAGTAT-G-ATAGAGG-AAAGCAGAA-TTTCTAGT-G-TAGA-G-GTG-AAAT-TCG-T-AG-A-TATTA-G-AAA-GAATACCA-ATTG-CGA-AGGCAGC-TTTCT-GGATCATTACTGACA-C-TGAGGA-ACG-AAAGC-AT-GGGTAGCG-AAG-AGGA

10 GC b GT-TGAAAAAA-GTT-GGTG-GTG-AAAT-CCCAGA-GCTTAA-CT-CTGGAAC-TG-CCATC-AAAAC-TTTTCAG-CTA-GAGTAT-G-ATAGAGG-AAAGCAGAA-TTTCTAGT-G-TAGA-G-GTG-AAAT-TCG-T-AG-A-TATTA-G-AAA-GAATACCA-ATTG-CGA-AGGCAGC-TTTCT-GGATCATTACTGACA-C-TGAGGA-ACG-AAAGC-AT-GGGTAGCG-AAG-AGGA

9 GC b GT-TG-AAAAA-GTT-GGTG-GTG-AAAT-CCCAGA-GCTTAA-CT-CTGGAAC-TG-CCATC-AAAACTTTTTCAG-CTA-GAGTAT-G-ATAGAGG-AAAGCAGAA-TTTCTAGT-G-TAGA-G-GTG-AAAT-TCG-T-AG-A-TATTA-G-AAA-GAATACCA-ATTG-CGA-AGGCAGC-TTTCT-GGATCATTACTGACA-C-TGAGGA-ACG-AAAGC-AT-GGGTAGCG-AAG-AGGA

7 GC b GT-TG-AAAAA-GTT-GGTG-GTG-AAAT-CCCAGA-GCTTAA-CT-CTGGAAC-TG-CCATCAAAAAC-TTTTCAG-CTA-GAGTAT-G-ATAGAGG-AAAGCAGAA-TTTCTAGT-G-TAGA-G-GTG-AAAT-TCG-T-AG-A-TATTA-G-AAA-GAATACCA-ATTG-CGA-AGGCAGC-TTTCT-GGATCATTACTGACA-C-TGAGGA-ACG-AAAGC-AT-GGGTAGCG-AAG-AGGA

5 GC b GT-TG-AAAAA-GTT-GGTG-GTG-AAAT-CCCAGA-GCTTAA-CT-CTGGAAC-TG-CCATC--AAAC-TTTTCAG-CTA-GAGTAT-G-ATAGAGG-AAAGCAGAA-TTTCTAGT-G-TAGA-G-GTG-AAAT-TCG-T-AG-A-TATTA-G-AAA-GAATACCA-ATTG-CGA-AGGCAGC-TTTCT-GGATCATTACTGACA-C-TGAGGA-ACG-AAAGC-AT-GGGTAGCG-AAG-AGGA

5 GC b GT-TG-AAAAA-GTT-GGTG-GTG-AAAT-CCCAGA-GCTTAA-CT-CTGGAAC-TG-CCATC-AAAAC-TTTTCAG-CTA-GAGTAT-G-ATAGAGG-AAAGCAGAA-TTTCTAGT-G-TAGA-G-GTG-AAAT-TCG-T-AG-A-TATTA-G-AAA-GAATACCA-ATTG-CGA-AGGCAGC-TTTCT-GGATCATTACTGACA-C-TGAGGA-ACG--AAGC-AT-GGGTAGCG-AAG-AGGA

5 GC b GT-TG-AAAAA-GTT-GGTG-GTG-AAAT-CCCAGA-GCTTAA-CT-CTGGAAC-TG-CCATC-AAAAC-TTTTCAG-CTA-GAGTAC-G-ATAGAGG-AAAGCAGAA-TTTCTAGT-G-TAGA-G-GTG-AAAT-TCG-T-AG-A-TATTA-G-AAA-GAATACCA-ATTG-CGA-AGGCAGC-TTTCT-GGATCATTACTGACA-C-TGAGGA-ACG-AAAGC-AT-GGGTAGCG-AAG-AGGA

5 GC b GT-TG-AAAAA-GTT-GGTG-GTG-AAAT-CCCAGA-GCTTAA-CT-CTGGAAC-TG-CCATC-AAAAC-TTTTCAG-CTA-GAGTAT-G-ATAGAGG-AAAGCAGAA-TTTCTAGT-G-TAGA-G-GTG-AAAT-TCG-T-AG-A-TATTA-G-AAA-GAACACCA-ATTG-CGA-AGGCAGC-TTTCT-GGATCATTACTGACA-C-TGAGGA-ACG-AAAGC-AT-GGGTAGCG-AAG-AGGA

4 GC b GT-TG-AAAAA-GTT-GGTG-GTG-AAAT-CCCAGA-GCTTAA-CT-CTGGAAC-TG-CCATC-AAAAC-TTTTCAG-CTA-GAGTAT-G-ATAGAGG-AAAGCAGAA-TTTCTAGT-G-TAGA-G-GTG-AAAT-TCG-T-AG-A-TATTA---GAA-GAATACCA-ATTG-CGA-AGGCAGC-TTTCT-GGATCATTACTGACA-C-TGAGGA-ACG-AAAGC-AT-GGGTAGCG-AAG-AGGA

4 GC b GT-TG-AAAAA-GTT-GGTG-GTG-AAAT-CCCAGA-GCTTAA-CT-CTGGAAC-TG-CCATC-AAAAC-TTTTCAG-CTA-GAGTAT-G-ATAGAGG-AAAGCAGAA-TTTCTAGT-G-TAGA-G-GTG-AAAT-TCG-T-AG-A-TATTA-G-AAA-GAATACCA-ACTG-CGA-AGGCAGC-TTTCT-GGATCATTACTGACA-C-TGAGGA-ACG-AAAGC-AT-GGGTAGCG-AAG-AGGA

4 GC b GT-TG-AAAAA-GTT-GGTG-GTG-AAAT-CCCAGA-GCTTAA-CT-CTGGAAC-TG-CCATC-AAAAC-TTTTCAG-CTA-GAGTAT-G-ATAGAGG-AAAGCAGAA-TTTCTAGT-G-TAGA-G-GTG-AAAT-CCG-T-AG-A-TATTA-G-AAA-GAATACCA-ATTG-CGA-AGGCAGC-TTTCT-GGATCATTACTGACA-C-TGAGGA-ACG-AAAGC-AT-GGGTAGCG-AAG-AGGA

4 GC b GT-TG-AAAAA-GTT-GGTG-GTG-AAAT-CCCAGA-GCTTAA-CT-CTGGAAC-TG-CCATC-AAAAC-TTTTCAG-CTA-GAGTAT-G-ATAGAGG-AAAGCAGAA-TTTCTAGT-G-TAGA-G-GTG-AAAT-TCG-T-AG-A-TATTA-G-AAA-GAATACCA-ATTG-CGA-AGGCGGC-TTTCT-GGATCATTACTGACA-C-TGAGGA-ACG-AAAGC-AT-GGGTAGCG-AAG-AGGA

4 GC b GT-TG-AAAAA-GTT-GGTG-GTG-AAAT--CCAGA-GCTTAA-CT-CTGGAAC-TG-CCATC-AAAAC-TTTTCAG-CTA-GAGTAT-G-ATAGAGG-AAAGCAGAA-TTTCTAGT-G-TAGA-G-GTG-AAAT-TCG-T-AG-A-TATTA-G-AAA-GAATACCA-ATTG-CGA-AGGCAGC-TTTCT-GGATCATTACTGACA-C-TGAGGA-ACG-AAAGC-AT-GGGTAGCG-AAG-AGGA

4 GC b GT-TG-AAAAAAGTT-GGTG-GTG-AAAT-CCCAGA-GCTTAA-CT-CTGGAAC-TG-CCATC-AAAAC-TTTTCAG-CTA-GAGTAT-G-ATAGAGG-AAAGCAGAA-TTTCTAGT-G-TAGA-G-GTG-AAAT-TCG-T-AG-A-TATTA-G-AAA-GAATACCA-ATTG-CGA-AGGCAGC-TTTCT-GGATCATTACTGACA-C-TGAGGA-ACG-AAAGC-AT-GGGTAGCG-AAG-AGGA

4 GC b GT-TG-AAAAA-GTT-GGTG-GTG-AAAT-CCCAGA-GCTTAA-CC-CTGGAAC-TG-CCATC-AAAAC-TTTTCAG-CTA-GAGTAT-G-ATAGAGG-AAAGCAGAA-TTTCTAGT-G-TAGA-G-GTG-AAAT-TCG-T-AG-A-TATTA-G-AAA-GAATACCA-ATTG-CGA-AGGCAGC-TTTCT-GGATCATTACTGACA-C-TGAGGA-ACG-AAAGC-AT-GGGTAGCG-AAG-AGGA

3 GC b GT-TG-AAAAA-GTT-GGTG-GTG-AAAT-CCCAGA-GCTTAA-CT-CCGGAAC-TG-CCATC-AAAAC-TTTTCAG-CTA-GAGTAT-G-ATAGAGG-AAAGCAGAA-TTTCTAGT-G-TAGA-G-GTG-AAAT-TCG-T-AG-A-TATTA-G-AAA-GAATACCA-ATTG-CGA-AGGCAGC-TTTCT-GGATCATTACTGACA-C-TGAGGA-ACG-AAAGC-AT-GGGTAGCG-AAG-AGGA

3 GC b GT-TG-AAAAA-GTT-GGTG-GTG-AAAT-CCCAGA-GCTTAA-CT-CTGGAAC-TG-CCATC-AAAAC-TTTTCAG-CTA-GAGTAT-G-ATAGAGG-AAAGCAGAA-TTTCTAGT-G-TAGA-G-GTG-AAAT-TCG-T-AG-A-TATTA-G-AAA-GAATACCA-ATTG-CGA-AGGCAGC-TTTCT-GGATCTTTACTGACA-C-TGAGGA-ACG-AAAGC-AT-GGGTAGCG-AAG-AGGA

3 GC b GT-TG-AAAAA-GTT-GGTG-GTG-AAAT-CCCAGA-GCTTAA-CT-CTGGAAC-TG-CCATC-AAAAC-TTTTCAG-CTA-GAGTAT-G-ATAGAGG-AAAGCAGAA-TTTCTAGT-G-TAGA-G-GTG-AAAT-TCG-T-AG-A-TATTA-G-AAA-GAATATCA-ATTG-CGA-AGGCAGC-TTTCT-GGATCATTACTGACA-C-TGAGGA-ACG-AAAGC-AT-GGGTAGCG-AAG-AGGA

BLAST Hits

» Alignments obtained for the most frequent sequence of GC are shown below.

Uncultured bacterium clone SPOT_10_4_885m_2_F_8_669.1 16S ribosomal RNA gene, partial sequence
Accession: KF227767
Identity : 100.0%
Coverage : 100.0%

QUERY: GTTGAAAAAGTTGGTGGTGAAATCCCAGAGCTTAACTCTGGAACTGCCATCAAAACTTTTCAGCTAGAGTATGATAGAGGAAAGCAGAATTTCTAGTGTAGAGGTGAAATTCGTAGATATTAGAAAGAATACCAATTGCGAAGGCAGCTTTCTGGATCATTACTGACACTGAGGAACGAAAGCATGGGTAGCGAAGAGGA
MATCH: ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
SUBJE: GTTGAAAAAGTTGGTGGTGAAATCCCAGAGCTTAACTCTGGAACTGCCATCAAAACTTTTCAGCTAGAGTATGATAGAGGAAAGCAGAATTTCTAGTGTAGAGGTGAAATTCGTAGATATTAGAAAGAATACCAATTGCGAAGGCAGCTTTCTGGATCATTACTGACACTGAGGAACGAAAGCATGGGTAGCGAAGAGGA

Uncultured bacterium clone SPOT_10_4_885m_2_A_12_667.7 16S ribosomal RNA gene, partial sequence
Accession: KF227674
Identity : 100.0%
Coverage : 100.0%

Uncultured marine bacterium clone J2-H9 16S ribosomal RNA gene, partial sequence
Accession: KF186014
Identity : 100.0%
Coverage : 100.0%

Uncultured marine bacterium clone J12-F8 16S ribosomal RNA gene, partial sequence
Accession: KF185780
Identity : 100.0%
Coverage : 100.0%

Uncultured marine bacterium clone J12-F3 16S ribosomal RNA gene, partial sequence
Accession: KF185775
Identity : 100.0%
Coverage : 100.0%

Uncultured marine bacterium clone J4-H7 16S ribosomal RNA gene, partial sequence
Accession: KF185409
Identity : 100.0%
Coverage : 100.0%

Uncultured bacterium clone 706-FE_0_01-27F_A12 16S ribosomal RNA gene, partial sequence
Accession: KC295020
Identity : 100.0%
Coverage : 100.0%

Uncultured bacterium clone 736-FE_0_31-27F_G04 16S ribosomal RNA gene, partial sequence
Accession: KC295019
Identity : 100.0%
Coverage : 100.0%

Uncultured bacterium clone 735-FE_0_30-27F_F04 16S ribosomal RNA gene, partial sequence
Accession: KC295018
Identity : 100.0%
Coverage : 100.0%

Uncultured bacterium clone 943_FE_4_46-27F_F06 16S ribosomal RNA gene, partial sequence
Accession: KC294989
Identity : 100.0%
Coverage : 100.0%

Uncultured bacterium clone 942_FE_4_45-27F_E06 16S ribosomal RNA gene, partial sequence
Accession: KC294988
Identity : 100.0%
Coverage : 100.0%

Uncultured bacterium clone FE_4_30 16S ribosomal RNA gene, partial sequence
Accession: KC294913
Identity : 100.0%
Coverage : 100.0%

Uncultured bacterium clone 787_FE_1_34-27F_B11 16S ribosomal RNA gene, partial sequence
Accession: KC294869
Identity : 100.0%
Coverage : 100.0%

Uncultured bacterium clone FE_1_57 16S ribosomal RNA gene, partial sequence
Accession: KC294828
Identity : 100.0%
Coverage : 100.0%

Uncultured bacterium clone FE_1_23 16S ribosomal RNA gene, partial sequence
Accession: KC294825
Identity : 100.0%
Coverage : 100.0%

Uncultured bacterium clone A7_98 16S ribosomal RNA gene, partial sequence
Accession: JQ732070
Identity : 100.0%
Coverage : 100.0%

Uncultured bacterium clone A0_80 16S ribosomal RNA gene, partial sequence
Accession: JQ731961
Identity : 100.0%
Coverage : 100.0%

Uncultured bacterium clone A0_77 16S ribosomal RNA gene, partial sequence
Accession: JQ731959
Identity : 100.0%
Coverage : 100.0%

Uncultured bacterium clone A0_63 16S ribosomal RNA gene, partial sequence
Accession: JQ731947
Identity : 100.0%
Coverage : 100.0%

Uncultured bacterium clone A0_30 16S ribosomal RNA gene, partial sequence
Accession: JQ731917
Identity : 100.0%
Coverage : 100.0%