» A summary of what happened.
Minimum Entropy Decomposition analysis was performed on 24,000 read from 24 samples for "sponge-1K" with MED pipeline version 0.1-alpha (available from http://oligotyping.org/MED).
» Meta
Library version | 0.1-alpha |
Run date | 03 Nov 14 23:25:00 |
End of run | 03 Nov 14 23:26:36 |
» Given Parameters
Min entropy for a component to be picked for decomposition | 0.0965 |
Perform entropy normalization heuristics | True |
Max number of discriminants to use for decomposition | 4 |
Min total abundance of oligotype in all samples | 0 |
Min substantive abundance of an oligotype (-M) | 5.0 |
Maximum variation allowed in each node (-V) | 4 nt |
Nodes agglomerated based on co-occurence patterns | |
Merge homopolymer splits | False |
Skip removing outliers | False |
Try to relocate outliers | False |
» Input Data
Number of sequences analyzed | 24,000 |
Number of samples found | 24 |
Number of characters in each alignment | 402 |
Average read length (without gaps) | 374 |
» Handling Outliers
Outliers removed due to -M | 1,759 |
Outliers removed due to -V | 583 |
Total number of outliers removed during the refinement | 2,342 |
Relocated outliers originally removed due to -M | |
Relocated outliers originally removed due to -V | |
Total number of relocated outliers | |
Final number of outliers due to -M | 1,759 |
Final number of outliers due to -V | 583 |
Final total number of outliers | 2,342 |
» Nodes
Number of sequences analyzed | 24,000 |
Number of sequences represented after quality filtering | 21,658 |
Number of raw nodes (before the refinement) | 287 |
Number of final nodes (after the refinement) | 287 |
» Files to analyze results further via third partry applications
Representative sequences per node | node-representatives.fa.txt |
Read distribution among samples table | read_distribution.txt |
Sample/oligotype abundance data matrix (percents) | matrix_percents.txt |
Sample/oligotype abundance data matrix (counts) | matrix_counts.txt |
Environment file | environment.txt |
Mapping file | sample_mapping.txt |
GEXF file for network analysis | network.gexf |
Basic topology of MED nodes | topology.gexf |
» Total number of reads for each sample that were analyzed.
» Deafult
» cluster_analysis
jaccard |
bray |
kulczynski |
canberra |
horn |
» nmds_analysis
jaccard |
bray |
kulczynski |
canberra |
horn |
» SOURCE
» nmds_analysis
jaccard |
bray |
kulczynski |
canberra |
horn |
» heatmap_analysis
jaccard |
bray |
kulczynski |
canberra |
horn |
» SPECIES